A Guide to Polyacrylamide Gel Electrophoresis and
2018-3-5 · Discontinuous buffer systems use a gel separated into two sections (a large-pore stacking gel on top of a small-pore resolving gel, Figure 2.2) and different buffers in the gels and electrode solutions (Wheeler et al. 2004) In gel electrophoresis, proteins do not all enter the gel matrix at the same time. Samples are loaded
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