Denaturing Polyacrylamide/Urea Gel Electrophoresis
2018-8-22 · denaturing electrophoresis as they may form an atypical pattern. However these usual discrepancies are normally acceptable for analysis of cDNA or other ssDNA in denaturing PAGE. 1. For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following: 10X TBE Buffer 4 ml 20% acrylamide/bisacrylamide 10 ml UREA 19.2 g (to 8 …
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