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polyacrylamide gel protocol in american samoa

  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    Transfer Protocol: Load the protein sample onto a 4-20% Tris-Glycine polyacrylamide gel and run until desired resolution is achieved. (The electrophoresis can be followed by using pre-stained molecular weight markers). Set up the Submerged Western Transfer

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    Transfer Protocol: Load the protein sample onto a 4-20% Tris-Glycine polyacrylamide gel and run until desired resolution is achieved. (The electrophoresis can be followed by using pre-stained molecular weight markers). Set up the Submerged Western Transfer Cassette as …

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  • Protocols - Western Transfer (Western Blot)

    The length of each DNA fragment is a function of the number of tandem repeats contained within it. The RFLP technique requires at least 10–50 ng of essentially undegraded DNA (10,000–20,000 bp) and isotopic detection to obtain a result; the time required for analysis may be in excess of one week ( 4 ).

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  • Running agarose and polyacrylamide gels

    Both polyacrylamide and agarose gels may be dried using this kit. Gels are placed between two moistened sheets of Gel Drying Film, clamped between the Gel Drying Frames provided and allowed to …

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  • Discontinuous Polyacrylamide Gel Electrophoresis of

    2014-1-1 · Here we will polymerize the acrylamide gel sandwiched between the patterned coverslip and the silanized coverslip. During detachment, the gel will stay attached to the silanized coverslip and the protein from the patterned coverslip will be transferred to the free surface of the acrylamide gel, resulting in a micropatterned acrylamide surface. •

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  • Gel Drying Kit Protocol - Promega

    Préparer la solution de polyacrylamide appropriée, conformément aux protocoles actuels de la biologie moléculaire ou en tant que figurant dans le tableau 1. Pour un gel d'acrylamide dénaturant de 20 cm x 22 cm x 1,5 mm, 60 ml de solution de gel et d'une 10,1 x 8,2 cm x 1 mm de gel de 5 ml solution de gel …

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  • Polyacrylamide Hydrogel Micropatterning -

    Load the desired volume (e.g. 35 µL) of samples per well in a 10% polyacrylamide gel. Run SDS-PAGE for 30 minutes at 200 V. Rinse gel twice with distilled water. Incubate gel in cold Transfer Buffer for 15 minutes. IV. Immunoblotting and Imaging; Assemble transfer sandwich as per instrument instruction and then transfer the proteins onto a PVDF membrane.

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  • Running agarose and polyacrylamide gels

    2011-6-17 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be run with or without a denaturant. Gels that are run without a denaturant are referred to as native gels.

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  • Gel Drying Kit Protocol - Promega

    The Gel Drying Kit provides a convenient and economical alternative to expensive and sometimes problematic gel dryers and vacuum systems. Both polyacrylamide and agarose gels may be dried using this kit. Gels are placed between two moistened sheets of Gel Drying Film, clamped between the Gel Drying Frames provided and allowed to dry overnight.

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  • Gel Drying Kit Protocol - Promega

    The Gel Drying Kit provides a convenient and economical alternative to expensive and sometimes problematic gel dryers and vacuum systems. Both polyacrylamide and agarose gels may be dried using this kit. Gels are placed between two moistened sheets of Gel Drying Film, clamped between the Gel Drying Frames provided and allowed to dry overnight.

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  • Western Blotting Protocol for Characterizing

    Load the desired volume (e.g. 35 µL) of samples per well in a 10% polyacrylamide gel. Run SDS-PAGE for 30 minutes at 200 V. Rinse gel twice with distilled water. Incubate gel in cold Transfer Buffer for 15 minutes. IV. Immunoblotting and Imaging; Assemble transfer sandwich as per instrument instruction and then transfer the proteins onto a ...

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  • Native Polyacrylamide Gel Electrophoresis - an

    Native polyacrylamide gel electrophoresis is performed using 6% acrylamide gels in Tris‐boric‐EDTA (8.9 mM Tris base, 8.9 mM boric acid, 0.2 mM Na 2 EDTA) buffer, pH 8, as described by Laemmli (1970).Staining for GSNOR activity is carried out using a modification of the method reported by Seymour and Lazarus (1989) and Fernández et al (2003).Gels are soaked in 0.1 M sodium phosphate, pH 7 ...

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  • Polyacrylamide Hydrogel Micropatterning -

    2014-1-1 · Here we have described a protocol of protein transfer on acrylamide gel that does not require any specific chemistry for the crosslinking of the ECM protein to the gel. Observations of the fluorescence on the template micropatterned substrate ( Fig. 6.2 A and B) clearly show that the proteins were efficiently transferred on the acrylamide gel ...

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  • Ultrashort Separation Length Homogeneous

    2019-12-12 · To realize efficient homogeneous electrophoretic immunoassays, we introduce discontinuous polyacrylamide gels that enable quantitative assay completion in separation lengths as short as 350 μm in <10 s. The discontinuous cross-linked gels reduce the required electrophoretic separation lengths and thereby significantly reduce the required applied electrical potentials needed to …

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  • Polyacrylamide Gel-Entrapped Maltase: An Excellent

    Western Blot Protocol. Cell Lysing Protocol: Remove the culture media by aspiration. Wash cells in the dish once with ice-cold PBS and aspirate off PBS. Add ice-cold NP40 Cell Lysis Buffer (RIPA Lysis Buffer: 25mM Tris-HCl pH7.5, 150mM NaCl, 1% NP-40, 1mM EDTA pH8.0.

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    Abstract. At present, the most polymorphic genetic markers are DNA regions composed of a variable number of tandem repeats (VNTRs) (1, 2).The detection of VNTRs is made possible by restriction fragment-length polymorphism (RFLP) analysis via Southern blotting ().The length of each DNA fragment is a function of the number of tandem repeats contained within it.

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  • Discontinuous Polyacrylamide Gel Electrophoresis of

    A luminescent detection system that offers sensitive detection of NanoLuc fusion proteins directly on a polyacrylamide gel, eliminating the need for antibody-based methods. Nano-Glo® In-Gel Detection System Protocol

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  • Nano-Glo® In-Gel Detection System Protocol

    Native polyacrylamide gel electrophoresis is performed using 6% acrylamide gels in Tris‐boric‐EDTA (8.9 mM Tris base, 8.9 mM boric acid, 0.2 mM Na 2 EDTA) buffer, pH 8, as described by Laemmli (1970).Staining for GSNOR activity is carried out using a modification of the method reported by Seymour and Lazarus (1989) and Fernández et al (2003).Gels are soaked in 0.1 M sodium phosphate, pH 7 ...

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  • Native Polyacrylamide Gel Electrophoresis - an

    Load the desired volume (e.g. 35 µL) of samples per well in a 10% polyacrylamide gel. Run SDS-PAGE for 30 minutes at 200 V. Rinse gel twice with distilled water. Incubate gel in cold Transfer Buffer for 15 minutes. IV. Immunoblotting and Imaging; Assemble transfer sandwich as per instrument instruction and then transfer the proteins onto a ...

    Get Price
  • Western Blotting Protocol for Characterizing

    2019-12-12 · To realize efficient homogeneous electrophoretic immunoassays, we introduce discontinuous polyacrylamide gels that enable quantitative assay completion in separation lengths as short as 350 μm in <10 s. The discontinuous cross-linked gels reduce the required electrophoretic separation lengths and thereby significantly reduce the required applied electrical potentials needed to …

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  • Ultrashort Separation Length Homogeneous

    2002-10-8 · Phosphorylation of proteins by kinases is central to many cellular processes, including signal transduction. Thus, assays to identify or characterize kinases are a key tool for research in this area. Kinase substrates can be incorporated into polyacrylamide gels and used to characterize kinase activity in mixed samples. This methodology can be adapted for the identification of novel kinase ...

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  • Polyacrylamide Gel-Entrapped Maltase: An Excellent

    Define polyacrylamide. polyacrylamide synonyms, polyacrylamide pronunciation, polyacrylamide translation, English dictionary definition of polyacrylamide. n. A water-soluble white solid polyamide, , related to acrylic acid.

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  • In-Gel Kinase Assay as a Method to Identify Kinase ...

    Western Blot Protocol. Cell Lysing Protocol: Remove the culture media by aspiration. Wash cells in the dish once with ice-cold PBS and aspirate off PBS. Add ice-cold NP40 Cell Lysis Buffer (RIPA Lysis Buffer: 25mM Tris-HCl pH7.5, 150mM NaCl, 1% NP-40, 1mM EDTA pH8.0.

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  • QIAquick Gel Extraction Kit - Qiagen

    2021-2-6 · The QIAEX II Handbook contains a protocol for Polyacrylamide Gel Extraction. A specialized User-Developed Protocol (QQ05) is available when using the QIAquick Gel Extraction Kit for this purpose. Both protocols require the preparation of a diffusion buffer and a disposable plastic column or syringe barrel containing a Whatman GF/C filter or ...

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  • PAGE & HPLC - Integrated DNA Technologies

    Polyacrylamide gel electrophoresis (PAGE) separates full-length product from shorter species based on electric charge. PAGE purification is most effective for unmodified oligos that only need truncated product removed. It substantially reduces the amount (mass) of final oligo product; however, the dramatic increase seen in purity justifies the ...

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  • Evaluation of MRI Findings After Polyacrylamide Gel ...

    2019-9-15 · Characteristics of Polyacrylamide Gel on MRI. Because polyacrylamide gel contains 95–97.5% water [2–4], it has high signal intensity on T2-weighted images and low signal intensity on T1-weighted images, similar to that of water on MRI.Thus, polyacrylamide gel is best depicted using a T2-weighted technique with or without fat suppression to delineate its location and extent.

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  • QIAEX II Gel Extraction Kit - QIAGEN

    The QIAEX II Gel Extraction Kit provides QIAEX II suspension together with binding and wash buffers, and a comprehensive handbook. QIAEX II Suspension is also sold separately. Protocols are provided for purification of DNA from agarose gels, solutions, and polyacrylamide gels.

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  • Cross-linking in Hydrogels - A Review

    2014-4-2 · American Journal of Polymer Science 2014, 4(2): 25-31 DOI: 10.5923/j.ajps.20140402.01 Cross-linking in Hydrogels - A Review Jaya Maitra*, Vivek Kumar Shukla Gautam Buddha University, Greater Noida, Gautam Budh Nagar-201312 (U.P), India

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  • Diamond™ Nucleic Acid Dye | Nucleic Acid Stain | Ethidium ...

    Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel. Lane 1: 10µl of BenchTop 1kb DNA Ladder (Cat.# G7541); lane 2–10: twofold serial dilutions of ladder in 1X Blue/Orange Loading Dye (Cat.# G1881). Following

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  • Western Blot Protocol | OriGene

    Western Blot Protocol. Cell Lysing Protocol: Remove the culture media by aspiration. Wash cells in the dish once with ice-cold PBS and aspirate off PBS. Add ice-cold NP40 Cell Lysis Buffer (RIPA Lysis Buffer: 25mM Tris-HCl pH7.5, 150mM NaCl, 1% NP-40, 1mM EDTA pH8.0.

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  • Jensen - American Association for Anatomy

    2012-2-3 · Transfer of Proteins. Once electrophoresis is complete, the separated proteins can be transferred from within the gel onto a membrane (a western blot) made of nitrocellulose, polyvinylidene difluoride, activated paper, or activated nylon (Towbin et al., 1979; Kurien and Scofield, 2006).Nitrocellulose is the most commonly used membrane.

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  • Polyacrylamide Hydrogel Micropatterning -

    2014-1-1 · Here we have described a protocol of protein transfer on acrylamide gel that does not require any specific chemistry for the crosslinking of the ECM protein to the gel. Observations of the fluorescence on the template micropatterned substrate ( Fig. 6.2 A and B) clearly show that the proteins were efficiently transferred on the acrylamide gel ...

    Get Price
  • Gibco-BRL V16 Polyacrylamide Gel Electrophoresis

    The Gel Drying Kit provides a convenient and economical alternative to expensive and sometimes problematic gel dryers and vacuum systems. Both polyacrylamide and agarose gels may be dried using this kit. Gels are placed between two moistened sheets of Gel Drying Film, clamped between the Gel Drying Frames provided and allowed to dry overnight.

    Get Price
  • Polyacrylamide Hydrogel: An Alternative Osteoarthritis ...

    2000-9-1 · The CT imaging is sensitive to radiation-induced density changes that occur within irradiated polyacrylamide gel (PAG). In this preliminary study, a CT imaging protocol is developed to optimize CT images of PAG; the response of PAG CT number to dose ( N CT -dose response) and the reproducibility of the response are investigated, and the use of ...

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  • Gel Drying Kit Protocol - Promega

    Western Blot Protocols (part 1) - Sample & Gel Preparation. Figure 1. Western blot experimental steps 1~5. From sample preparation to protein electrophoresis. 1. Sample preparation. Sample preparation is the first step and one of the most important steps of western blot. A good sample preparation makes your western blot half success.

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  • Polymer gel dosimetry using x-ray computed

    Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system, such as by UV transillumination with a Polaroid® or digital camera, GE ImageQuant™ or Bio-Rad Gel Doc™ systems. The concentrated dye is stable for up to 90 days at room temperature.

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  • Western Blot Protocols part 1 - Creative Diagnostics

    You can increase the percentage of the crosslinker in the regular 17% gel. Also raise the pH of your resolving gel to 9.5 as compared to your regular 8.8. Plus, the addition of urea (4-8M) helps sharpen bands. If you are going to use western, which is a way more sensitive detection method, please use Western instead of the gel staining.

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  • Diamond™ Nucleic Acid Dye | Nucleic Acid Stain |

    2012-7-26 · Load the samples on the polyacrylamide gel with control oligo sample (mixed upper and lower markers) on either side of the RNA samples. 22 Separate by electrophoresis for 65 min at 200 V.

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  • Evaluation of MRI Findings After Polyacrylamide Gel ...

    2019-9-15 · Characteristics of Polyacrylamide Gel on MRI. Because polyacrylamide gel contains 95–97.5% water [2–4], it has high signal intensity on T2-weighted images and low signal intensity on T1-weighted images, similar to that of water on MRI.Thus, polyacrylamide gel is best depicted using a T2-weighted technique with or without fat suppression to delineate its location and extent.

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  • AES Electrophoresis Society: Two-Dimensional Gel ...

    Figure 1. Two-dimensional polyacrylamide gel electrophoresis. Proteins from a lysate of Escherichia coli were subjected to IEF in a 17-cm IPG strip spanning the pH range of 4-to-7. The strip containing focused proteins was transferred to an 18 x 20 cm SDS-PAGE gel (8-16%T) and subjected to electrophoresis.

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  • Polyacrylamide Hydrogel Micropatterning -

    2014-1-1 · Here we have described a protocol of protein transfer on acrylamide gel that does not require any specific chemistry for the crosslinking of the ECM protein to the gel. Observations of the fluorescence on the template micropatterned substrate ( Fig. 6.2 A and B) clearly show that the proteins were efficiently transferred on the acrylamide gel ...

    Get Price
  • QIAquick Gel Extraction Kit - Qiagen

    2021-2-6 · The QIAEX II Handbook contains a protocol for Polyacrylamide Gel Extraction. A specialized User-Developed Protocol (QQ05) is available when using the QIAquick Gel Extraction Kit for this purpose. Both protocols require the preparation of a diffusion buffer and a disposable plastic column or syringe barrel containing a Whatman GF/C filter or ...

    Get Price
  • The principle and method of Western blotting (WB) |

    After SDS-PAGE, a membrane is placed on the gel, to which the separated proteins in the gel are electrophoretically transferred. The membrane with transferred proteins is then probed with a primary antibody (an antibody specific for the target protein), washed, and reacted with a secondary antibody labeled with an enzyme, such as horseradish peroxidase (HRP).

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  • Mouse Anti-Human Aldo-Keto Reductase Family 1

    Protocol; Product name Mouse Anti-Human Aldo-Keto Reductase Family 1 Member C1 Catalog number 128-10228-1 (5ug) 128-10228-2 (20ug) 128-10228-3 (100ug) Description Mouse Anti-Human Aldo-Keto Reductase Family 1 Member C1 Source Raybiotech Product category Antibodies Product sub category Primary Antibodies Target species Human Target's name

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  • Southwestern blotting in investigating transcriptional ...

    2007-12-20 · Southwestern blotting is a technique developed for the detection and characterization of protein factors interacting with distinct DNA motifs. The technique is modified from a …

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  • Acrylamide gels | definition of Acrylamide gels by

    polyacrylamide gel: n. A hydrated polymer consisting of a long chain of amide groups, used as a medium for substances that undergo gel electrophoresis.

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  • Taq PCR Master Mix Kit - QIAGEN

    Taq PCR Master Mix includes Taq DNA Polymerase, QIAGEN PCR Buffer, MgCl 2, and ultrapure dNTPs at optimized concentrations.The ready-to-use 2x master mix format minimizes pipetting errors and also provides greater convenience. PCR setup is fast, easy, and straightforward — only primers and template DNA need to be added.

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  • Sodium dodecyl sulfate-polyacrylamide

    2010-4-12 · The polyacrylamide gel is a cross-linked matrix that functions as a sort of sieve to help 'catch' the molecules as they are transported by the electric current. The polyacrylamide gel acts somewhat like a three-dimensional mesh or screen. The negatively charged protein molecules are pulled to the positive end by the current, but they encounter ...

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  • Cross-linking in Hydrogels - A Review

    2014-4-2 · American Journal of Polymer Science 2014, 4(2): 25-31 DOI: 10.5923/j.ajps.20140402.01 Cross-linking in Hydrogels - A Review Jaya Maitra*, Vivek Kumar Shukla Gautam Buddha University, Greater Noida, Gautam Budh Nagar-201312 (U.P), India

    Get Price
  • The principle and method of Western blotting (WB) |

    After SDS-PAGE, a membrane is placed on the gel, to which the separated proteins in the gel are electrophoretically transferred. The membrane with transferred proteins is then probed with a primary antibody (an antibody specific for the target protein), washed, and reacted with a secondary antibody labeled with an enzyme, such as horseradish peroxidase (HRP).

    Get Price
  • Effect of prehabilitation on the outcome of anterior ...

    Background: Prehabilitation is defined as preparing an individual to withstand a stressful event through enhancement of functional capacity. Hypothesis: We hypothesized that a preoperative exercise program would enhance postoperative outcomes after anterior cruciate ligament reconstruction (ACLR). Study design: Randomized controlled clinical trial; Level of evidence, 1.

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  • Gel Electrophoresis Equipment Selection Guide |

    2 天前 · Polyacrylamide gel electrophoresis (PAGE) is one of the most common methods for separation of both nucleic acids and proteins, most often by mass. As its name implies, PAGE uses a polyacrylamide gel that is ideal for protein separation but also provides high resolving power for small DNA fragments.

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  • Current Protocols Essential Laboratory Techniques -

    The Essential Laboratory Techniques section provides overviews covering good laboratory practices, professional development guides, and resources to be used in the undergraduate laboratory to equip the novice researcher with a solid life science skill set.

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  • Glycerol for molecular biology, ≥99.0% | 56-81-5

    Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

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  • The FokI Restriction-Modification System

    2020-11-9 · THE JOURNAL OF BIOLOGICAL CHEMISTRY 0 1989 by The American Society for Biochemistry and Molecular Biology, Inc Vol. 264, No. Issue of April 5, pp. 5757-5761,1969 Printed in U. SA. The FokI Restriction-Modification System 11. PRESENCE OF TWO DOMAINS IN FokI METHYLASE RESPONSIBLE FOR MODIFICATION OF DIFFERENT DNA STRANDS* (Received for publication, …

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  • Clostridioides difficile (Prevot) Lawson et al. | ATCC

    Presence of tcdB gene confirmed by PCR.RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303Binary toxin gene cdtB not amplified by PCRToxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL …

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  • Why does the observed protein molecular weight

    2019-6-4 · 4th June 2019. Figure 1: Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1) (66248-1-Ig) is a type I transmembrane protein, acting as a key regulator of the adaptive immune response. Full-length PD-L1 molecular weight is 33 kDa. The signal peptide is cleaved off during protein transport to the plasma membrane, and the protein is heavily N ...

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  • Simple Method for Repurification of Endotoxins for ...

    2020-12-17 · A method for obtaining highly purified endotoxin (lipopolysaccharide [LPS]) in a few hours by repurification of commercial or laboratory preparations was devised. It avoids the use of phenol, which is not suitable for phenol-soluble lipopolysaccharides nor for some industrial purposes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization ...

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  • 'In-gel' purified ditags direct synthesis of highly ...

    2002-8-1 · Results. Ditags used to generate SAGE libraries were demarcated 'in situ' on preparative polyacrylamide gels using XC and BPB dyes, which precisely straddle the ditag band when a 16% PAGE gel (19:1 acrylamide:bis, 5% cross linker) is used to resolve the DNA bands.Here, the ditag DNA was directly excised from gel without visualization via EtBr or fluorescent dye staining, resulting in highly ...

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  • VitroGel® RGD hydrogel for 3D cell culture | TheWell ...

    2021-7-10 · VitroGel® RGD High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel RGD High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.

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  • RESEARCH Open Access Alternate day fasting for weight

    2017-8-27 · [10,11]. High-resolution 3% polyacrylamide gel tubes were used for electrophoresis. Briefly, 25μLofsample was mixed with 200μL of liquid loading gel containing Sudan black, and added to the gel tubes. After photo-polymerization at room temperature for 30 min, samples were electrophoresed for 1 h (3 mA/gel tube). Lipoware

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  • Gel Shift Assay Systems - Promega

    The gel shift assay involves incubating a purified protein or a complex mixture of proteins (such as nuclear or cell extract preparations) with a 32 P end-labeled DNA fragment containing the putative protein binding site. The reaction products are then analyzed on a nondenaturing polyacrylamide gel.

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  • A Standardized and Reproducible Urine Preparation

    2019-4-12 · Symptoms in infected plants were similar to those produced by Ornithogalum mosaic virus (OMV). An enzyme-aided purification protocol was developed, which eliminated a highly viscous mucilage from extracts of both species. Serological tests indicated that virus isolates from Ornithogalum (OMV-O) and from Lachenalia (OMV-L) were indistinguishable.

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  • COVID-19 advice - Protect yourself and others | WHO ...

    4647 Superior Street P.O. Box 4425 Lincoln, NE 68504-0425 United States

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  • Phytopathology 1989 | Purification and Some

    2021-7-12 · The E.Z.N.A.® Bacterial DNA Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of gram-positive and negative bacterial species. This kit uses an optimized lysis condition and up to 1 x 10^ 9 bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic ...

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