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    As you would expect from the name, DGGE works by using a gradient of denaturing strength along either the vertical or horizontal axis of a polyacrylamide gel. DNA samples from various sources are amplified by PCR with identical primers and then electrophoresed in this gel, similar to standard polyacrylamide gel electrophoresis. …

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    SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field.It uses sodium dodecyl sulfate (SDS) molecules to help identify and isolate …

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    2021-6-23 · Boyuan is one of the most professional polyacrylamide manufacturers and suppliers in China. Our factory offers high quality polyacrylamide made in China with competitive price. Welcome to place an order.

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    Qingdao Oubo Chemical Co., Ltd. was established in 2011, we specialize in supplying cationic polyacrylamide, anionic polyacrylamide and nonionic polyacrylamide. Send Inquiry Polyacrylamide nonionic water-soluble polymer Sigma

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    Most acrylamide is used to synthesize polyacrylamides, which find many uses as water-soluble thickeners. These include use in wastewater treatment, gel electrophoresis (SDS-PAGE), papermaking, ore processing, and the manufacture of permanent press fabrics. Some acrylamide is used in the manufacture of dyes and …

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    2009-12-8 · Results. The correlations between the Friedewald equation and the 4 homogeneous LDL-C assays were as follows: LDL-C (L) (r = 0.962), LDL-C (S) (r = 0.986), LDL-C (E) (r = 0.946) and LDL-C (P) (r . = 0.963).Discrepancies were observed in sera from type III hyperlipoproteinemia patients and in sera containing large amounts of midband and small dense LDL on polyacrylamide gel electrophoresis.

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    Polyacrylamide gel electrophoresis in quantification of high-density lipoprotein cholesterol Clin Chem. 1985 Nov;31(11):1893-5. ... sample through a discontinuous polyacrylamide++ gel and densitometric integration of the stain associated with each class of lipoprotein. With this method, operations can be carried out on all types of …

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    2016-7-22 · Polyacrylamide gel electrophoresis Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a free-radical polymerization that requires an initiator, usually ammonium …

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    Abstract. Since O’Farrell introduced the improved technique for high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), it has become one of the most powerful tools for the separation and quantification of proteins from complex mixtures.The major reason for this success is that 2-D PAGE employs separation …

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    Quantification of Rat Neurophysins by Polyacrylamide Gel Electrophoresis (page): Application to the Rat with Hereditary Hypothalamic Diabetes Insipidus Sunde, David A. Sokol, Hilda Weyl

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    Nonionic Polyacrylamide Pam Buy Nonionic. Nonionic Polyacrylamide Details:. PAM is a water-soluble polymer, the appearance of a white or yellowish powder, easy to absorb water, long-term thermal decomposition, the decomposition temperature above 200 ℃, under anaerobic conditions, 210 ℃ carbonized black powder.

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    Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, …

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    2021-6-23 · Nonionic Polyacrylamide. It is mainly used for flocculation, sedimentation and clarification of various industrial wastewater. Read More. ... As one of the most professional polyacrylamide manufacturers and suppliers in China, we're featured by quality products and good service. Please rest assured to wholesale bulk polyacrylamide …

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    Blue native‐polyacrylamide gel electrophoresis (BN‐PAGE) The supernatant enriched with mitochondrial complexes was supplemented with a 5% (w/v) stock solution of Serva Blue G in solubilization medium to a final ratio of 1:4 (w:w) of dye to detergent, and subjected to BN‐PAGE according to ( Schägger and von Jagow, 1991 ).

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    2016-3-23 · Polyacrylamide Gel Electrophoresis Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylene-bis-acrylamide, commonly called BIS. This process is a free-radical polymerization that requires an initiator, usually ammonium

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    China Polyacrylamide Gel Electrophoresis,Anionic. Polyacrylamide is a kind of high molecular polymer. It is widely used for industry like water treatment, paper, oil, coal, mine, textile, construction, etc. China leading manufacturers and suppliers of Polyacrylamide Gel Electrophoresis and we are specialize in Anionic Polyacrylamide, Polyacrylamide Crystals, Polyacrylamide …

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    Polyacrylamide Gel Electrophoresis Market Insights 2019, Global and Chinese Scenario is a professional and in-depth study on the current state of the global Polyacrylamide Gel Electrophoresis industry with a focus on the Chinese market. The report provides key statistics on the market status of the Polyacrylamide Gel Electrophoresis …

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    2020-3-24 · 1 EVALUATION AND RECOMMENDATION OF PHARMACOPOEIAL TEXTS FOR USE IN THE ICH REGIONS ON POLYACRYLAMIDE GEL ELECTROPHORESIS GENERAL CHAPTER Q4B ANNEX 10(R1) 1. INTRODUCTION This annex is the result of the Q4B process for the Polyacrylamide Gel Electrophoresis General Chapter. The …

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    2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. In most denaturing agarose gel systems bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA.

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  • Two-dimensional polyacrylamide gel

    2018-5-16 · Another advancement in 2-D gel separations was introduced in 1972 by Wright , who used a 4.75% (2% cross-linkage) polyacrylamide gel column in the first dimension, which was then removed from the glass cylinder and laid on the upper edge of a 2% gradient slab. Following electrophoresis, the gel slab was placed in a …

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  • Separating Protein: SDS-Polyacrylamide Gel

    Poly-Acrylamide Gel Electrophoresis utilizes a hydrogel made from polyacrylamide. Polyacrylamide is a polymer that forms a very regular matrix through which proteins can move. The more concentrated the gel is, the slower the proteins will traverse across it when exposed to an electric field.

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  • China Anionic Polyacrylamide Emulsion

    2021-7-9 · Getting started; Anionic Polyacrylamide Emulsion; Anionic Polyacrylamide Emulsion - Manufacturers, Factory, Suppliers from China. Fast and great quotations, informed advisers to help you choose the correct solution that suits all your requirements, a short creation time, responsible top quality manage and distinct providers for paying and shipping affairs for Anionic Polyacrylamide Emulsion …

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    2008-1-12 · In proteomics, one-dimensional (1D) sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE) is widely used for protein fractionation prior to mass spectrometric analysis to enhance the dynamic range of analysis and to improve the identification of low-abundance proteins. Such protein …

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  • Polyacrylamide Gel Electrophoresis | Science

    Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, …

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    Polyacrylamide polymer msds Polyacrylamide is a kind of linear polymers,it can soluble in water,insoluble in most organic solvents. According to the product features can be divided into:anionic polyacrylamide,cationic polyacrylamide,and non-ionic polyacrylamide. it is widely used in oil recovery,mineral processing,coal …

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  • Two-Dimensional Polyacrylamide Gel Electrophoresis

    2018-9-25 · Two-Dimensional Polyacrylamide Gel Electrophoresis A Practical Perspective 95 The 2-D electrophoresis, especially IEF in the first dimension, is very sensitive to many interfering compounds including lipids, nucleic acids, and small ionic molecules. These contaminants can be eliminated by additional steps such as …

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    Most acrylamide is used to synthesize polyacrylamides, which find many uses as water-soluble thickeners. These include use in wastewater treatment, gel electrophoresis (SDS-PAGE), papermaking, ore processing, and the manufacture of permanent press fabrics. Some acrylamide is used in the manufacture of dyes and …

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    Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. BACKGROUND. SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used in biochemistry and molecular biology to separate proteins according to their electrophoretic mobility (a function of length of polypeptide chain or molecular …

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    Polyacrylamide nonionic water-soluble polymer Sigma-Aldrich. Polyacrylamide is used in the preparation of acrylamide gels for separation of proteins. Biochem/physiol Actions Polyacrylamide is a water-soluble polymer made up of acrylamide subunits. It increases the viscosity of water and facilitates the flocculation of particles present …

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    2017-7-11 · Rainbow Molecular Weight Markers are available in three size ranges for use with polyacrylamide gel electrophoresis (SDS-PAGE). Full-, High-, and Low-Range (left to right). Rainbow molecular markers are available for both chemiluminescence based detection and fluorescence detection applications.

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    Most acrylamide is used to synthesize polyacrylamides, which find many uses as water-soluble thickeners. These include use in wastewater treatment, gel electrophoresis (SDS-PAGE), papermaking, ore processing, and the manufacture of permanent press fabrics. Some acrylamide is used in the manufacture of dyes and …

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    SDS-PAGE is an electrophoresis method that allows protein separation by mass. The medium (also referred to as ′matrix′) is a polyacrylamide-based discontinuous gel. In addition, SDS (sodium dodecyl sulfate) is used. About 1.4 grams of SDS bind to a gram of protein, corresponding to one SDS molecule per two amino acids.

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    polyacrylamide gel electrophoresis, etc ; milk; sulfates; two-dimensional gel electrophoresis; whey; Show all 5 Subjects Abstract: Over the years, a number of methodological variations in polyacrylamide gel electrophoresis (PAGE) have been attempted for the separation of milk proteins.

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  • Preparation of protein samples for SDS

    2006-6-25 · Preparation of protein samples for SDS-polyacrylamide gel electrophoresis: procedures and tips Anthony C. Grabski 1and Richard R. Burgess2— Novagen, Inc. and 2McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706 continued from page 9 pensive method …

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  • Sodium Dodecyl Sulphate Polyacrylamide Gel

    2 天前 · As an analytical tool, electrophoresis is simple, rapid and highly sensitive. It is used analytically to study the properties of a single charged species, and as a separation technique. Support Matrices. Generally the sample is run in a support matrix such as paper, cellulose acetate, starch gel, agarose or polyacrylamide gel.

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    2021-7-7 · Polyacrylamide gel electrophoresis (PAGE) is a typical technique for protein separation by electrophoresis.In this technique, polyacrylamide gel is used as the base medium, while sodium dodecyl sulfate (SDS) is used for protein chain and protein denaturation. Market Analysis and Insights: Global ...

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  • Gel Electrophoresis - .NET Framework

    2017-9-25 · Polyacrylamide Gel Electrophoresis (PAGE) Polyacrylamide gel is the result of polymerizing acrylamide monomers into long chains and then cross-linking the chains with a bifunctional compound. A number of these bifunctional cross-linkering compounds are known including ethylene diacrylate, N,N’-

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    Gel electrophoresis technique has been widely used in many area in biotechnology includes molecular biology, biochemistry, genetics and forensics.The result of gel electrophoresis is often incorporated with other techniques depends on the type of analysis being carried out and thus provided a large range of field-specific …

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  • SDS-polyacrylamide gel electrophoresis (SDS

    electrophoresis (ĭlĕk'trōfərē`sĭs): see colloidcolloid [Gr.,=gluelike], a mixture in which one substance is divided into minute particles (called colloidal particles) and dispersed throughout a second substance. The mixture is also called a colloidal system, colloidal solution, or colloidal dispersion. ..... Click the link for more …

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    SDS-polyacrylamide gel electrophoresis (SDS-PAGE) synonyms, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) pronunciation, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) translation, English dictionary definition of SDS-polyacrylamide gel electrophoresis (SDS-PAGE). ... medical, or any other professional…

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    The hydrated PAM is a soft gel that is used in gel electrophoresis and as a super water-absorbing polymer (SAP's). Even though these polymers are called polyacrylamide, they are often copolymers of acrylamide and one or more other monomers. ... Anionic Polyacrylamide, Cationic polyacrylamide, Nonionic. Qingdao …

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    2015-1-1 · Quantification of OMV yields is a key step in the understanding of vesiculation. Relative quantification is also useful after density gradient centrifugation or gel filtration to find out which fractions are OMV-rich and are to be combined for further processing. The quantification is based either on the measurements of …

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    Polyacrylamide Gel Electrophoresis. Michael R. Green and Joseph Sambrook; Abstract. Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the …

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    2006-6-25 · Preparation of protein samples for SDS-polyacrylamide gel electrophoresis: procedures and tips Anthony C. Grabski 1and Richard R. Burgess2— Novagen, Inc. and 2McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706 continued from page 9 pensive method …

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    Polyacrylamide Gel Electrophoresis has a number of advantages, which are: PAGE has a high loading capacity, up to 10 micrograms of DNA can be loaded into a single well (1 cm x 1 mm) without significant loss of resolution. Polyacrylamide contains few inhibitors of enzymatic reactions.

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    2021-6-2 · [Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Objectives: Gel Electrophoresis: Electrophoresis is the movement of molecules by an electric current. Nucleic acid moves from a negative to a positive pole and when DNA is applied to a macromolecular cage or gel such as agarose or polyacrylamide, its …

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    2016-3-2 · Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAGE) is yet another electrophoretic method for the detection of recombinant erythropoietin in samples of athletes. 3 Isoelectric focusing of EPO in polyacrylamide gel followed by double immunoblotting and chemiluminescent detection was the first method, …

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    Nanoparticles (NPs) are able to improve the separation efficiency of proteins in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) due to their capability of enhancing heat dissipation during electrophoresis. However, the intrinsic surface charges of NPs (at buffer pH or charge induced due to the SDS coating

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    The hydrated PAM is a soft gel that is used in gel electrophoresis and as a super water-absorbing polymer (SAP's). Even though these polymers are called polyacrylamide, they are often copolymers of acrylamide and one or more other monomers. The most important co-monomer is acrylic acid or sodiumacrylate. …

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    2021-5-24 · Zwitter-ion polyacrylamide_OKCHEM. Zwitter-ion polyacrylamide 9003-05-8,Can be used as flocculant, coagulant for sludge dewatering, chemical for treatment of special sludge and mixed organic wastewater, adsorbent, metal ion chelant, papermaking additive and oilfield chemical

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    PAM Polyacrylamide|CPAM|APAM. Polyacrylamide is a polymer (-CH2CHCONH2-) formed from acrylamide subunits, it is long-chain polymer (same molecule repeating itself many times) designed to attract either positively charged particles (organic materials, such as carbon or human waste) or negatively charged particles (inert …

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    2021-7-9 · Getting started; Polydmdaac; Polydmdaac - Factory, Suppliers, Manufacturers from China. Now we have superior devices. Our solutions are exported to your USA, the UK and so on, enjoying a superb name between customers for Polydmdaac, Black Color Removal Chemical, Water Cleaning Chemical Polyamine, …

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    2018-9-7 · High molecular weight (106–3 × 107 Da) polyacrylamide (PAM) is commonly used as a flocculant in water and wastewater treatment, as a soil conditioner, and as a viscosity modifier and friction ...

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    2015-11-29 · Polyacrylamide gel electrophoresis (PAGE) is used for separating proteins ranging in size from 5 to 200 kiloDalton due to the uniform pore size provided by the polyacrylamide gel. Pore size is controlled by controlling the concentrations of acrylamide and bis- acrylamide powder used in creating a gel.

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    The second step in a Western blot is the “running of the gel” in which you load your samples onto a gel matrix and then use electricity to push and pull the proteins through the gel. Electrophoresis of proteins through the gel matrix separates the proteins prior to transfer of the proteins to a membrane support.

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    The following protocol is easily preformed with the Processor Plus or can be carried out manually using trays on a shaker. Process the gel (s) according to Table 4.1. Use 250 ml of each solution per gel for standard (14 × 16 cm) gels. Use 100 ml of each solution per gel for mini-format (8 × 10 cm) gels.

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    TABLE CONTENTS Part I: Theory and Product Selection 5 Chapter 1 Overview 5 How Protein Electrophoresis Works 6 General Considerations and Workflow 6 Chapter 2 Protein Electrophore

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    Ph.Eur. 2.2.31. Electrophoresis entitled “Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)”, JP General Information 23. SDS-Polyacrylamide Gel Electrophoresis, and USP <1056> Biotechnology-derived Articles – Polyacrylamide Gel Electrophoresis, can be used as interchangeable in the ICH …

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    Hoefer sds polyacrylamide gel electrophoresis sds page Sds Polyacrylamide Gel Electrophoresis Sds Page, supplied by Hoefer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

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    2 天前 · As an analytical tool, electrophoresis is simple, rapid and highly sensitive. It is used analytically to study the properties of a single charged species, and as a separation technique. Support Matrices. Generally the sample is run in a support matrix such as paper, cellulose acetate, starch gel, agarose or polyacrylamide gel.

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    2013-8-19 · Overall 1 × 10 6 cells were lysed, the protein concentration was determined by BCA assay (Pierce) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE) analysis with 25 µg of total protein was performed with NuPAGE gels (Invitrogen). Protein transfer to a nitrocellulose membrane was done …

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    2005-6-7 · The analysis of hydrophobic membrane proteins by two-dimensional gel electrophoresis has long been hampered by the concept of inherent difficulty due to solubility issues. We have optimized extraction protocols by varying the detergent composition of the solubilization buffer with a variety of commercially available non-ionic …

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